RDEC h4-1 ThMSC1 imabayashi 自分たちが論文で出した細胞名とウェブ上で公開されているデータベースにおける細胞名が異なる件。

今林氏の論文でのTable 4（以下）と、ncbiのウェブ上に掲載されている情報（その下）が同じである件。

細胞名は違うけど内容は一緒。ThMSC1 (UEET-1)というのと N1ESE2 というのが一緒なんだな。それで、トリコスタチンと5-azacytidineで処理した細胞は今林論文には含まれていないんだ。

今林論文 imabayashi は、

https://sites.google.com/site/marrowstroma/Home/paper-list/nishino-plos-one-2010/ImabayashiECR2003.pdf?attredirects=0&d=1

https://www.researchgate.net/publication/10647396

cDNA libraryのサイトは、

http://www.ncbi.nlm.nih.gov/UniGene/lbrowse2.cgi?TAXID=9606

Lib.12670	T1ESE2	2527
Lib.12664	N1ESE2

ncbiのサイトは

無処理のUEET-1細胞の発現データ

http://www.ncbi.nlm.nih.gov/UniGene/library.cgi?ORG=Hs&LID=12664

5-azacytidineとtrichostatin処理した発現データ

http://www.ncbi.nlm.nih.gov/UniGene/library.cgi?ORG=Hs&LID=12670

Table 4
The 15 most highly expressed genes in the cDNA library for RDEC, H4-1 (BMSC), and ThMSC1 cellsa

RDEC1のトップ３

Biglycan
Transforming growth factor, beta-induced

Cartilage oligomeric matrix protein

H4-1 (BMSC)のトップ３

Insulin-like growth factor binding protein-3

Transforming growth factor, beta-induced
Eukaryotic translation elongation factor-1 alpha 1

ThMSC1のトップ３

Insulin-like growth factor binding protein 3

Transforming growth factor, beta-induced

Vimentin

T1ESE2

Library Description

Organism:	Homo sapiens
Cell type:	mesenchymal stem cells
Vector:	pME18SFL3

mRNA from mesenchymal stem cells treated with trichostatin and 5-azacytidine

Source: Takao Isogai, Helix Research Institute

Gene Content Analysis

2,490 ESTs from this library were grouped into 1,217 UniGene entries (putative genes) [UniGene build #234, 22-Sep-2012]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM UniGene Entry

80	32129	http://www.ncbi.nlm.nih.gov/genome/guide/corehtml/Dot10.gif	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
63	25301	http://www.ncbi.nlm.nih.gov/genome/guide/corehtml/Dot10.gif	Hs.369397	Transforming growth factor, beta-induced, 68kDa.
62	24900	http://www.ncbi.nlm.nih.gov/genome/guide/corehtml/Dot10.gif	Hs.691131	Vimentin.

N1ESE2

Library Description

Organism:	Homo sapiens
Cell type:	mesenchymal stem cells
Vector:	pME18SFL3

Source: Takao Isogai, Helix Research Institute

Gene Content Analysis

2,429 ESTs from this library were grouped into 1,048 UniGene entries (putative genes) [UniGene build #234, 22-Sep-2012]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM UniGene Entry

114	46933	http://www.ncbi.nlm.nih.gov/genome/guide/corehtml/Dot10.gif	Hs.450230	Insulin-like growth factor binding protein 3.
111	45698	http://www.ncbi.nlm.nih.gov/genome/guide/corehtml/Dot10.gif	Hs.369397	Transforming growth factor, beta-induced, 68kDa.
81	33347	http://www.ncbi.nlm.nih.gov/genome/guide/corehtml/Dot10.gif	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.