Two MSCs lead cell-based therapy

Challenge with Mesenchymal Stem Cells and Marrow Stromal Cells

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Killer phrases and plus

Google search: Killer phrases.




Plus.
1. One subject at a time.
2. No counterproposal is yes.
3. What, By whom, By when.

Ayumi Y

One man's Systemization of HDK1 on immortalized HFFs.
1. Banking of Ay-HDK1-MCB (10 tubes), -MWCB (x 10), and -WCB (x 10).
2. Banking of Ay-iHFF-MCB (10 tubes), -MWCB (x 10), and -WCB (x 10).
3. SOP
4. Characteristics
5. Specification
        6. Record with Yoriko
        

HDK1 on immortalized HFFs in 3D condition.
Yugawa method.
Epidermal differentiation of XPA-, XPC- and GEN1-iPSCs.

Ando

ESC-derived MSC-knst

Bank of irradiated RMD9: Frozen stock 30 tubes.

iPSC: MRCiPS#25 and EdomiPS#22
No cartilage was obtained.

Manufacturing 
1. knst-MCB, -WCB from research bank of pHAES-WCB
2. Propagation in Pb10.

Target disease: 1. Fabry disease, 2. Ischemic limb, 3. Ischemic heart.

Product. CellSaic: RCP and knst



PLoS Med. 2005 Jun;2(6):e161.

Derivation of multipotent mesenchymal precursors from human embryonic stem cells.





Realization of Clinical trial or study (Mission: FIH for an endpoint of safety)
1. Product: Sheet or single forms of CellSaic with RCP and knst.
        A. Starting material: Bank of pHAES-rWCB or bFF-MCB
2. POC in vivo: Ask Hiroshi Suzuki.
        A. Target disease: Ischemic heart disease and Fabry heart. Ischemic limb. With FF. Like Chg-Fkwts.

Manufacturing
1. Generation of knst-rMCB
    A. Generation of irradiated RMD9 feeder cells
        a. Feeder cells (irrRMD9) are prepared from RMD9 as irrMEFs.
    B. Cells from pHAES-rWCB is propagated on irrRMD9.
    

Strength
1. The CMC of the product is partly established.

Issues
1. Unclear ingredients of knst like HeartSheet.
2. knst really works like HeartSheet.
3. Competitors.
4. Milestones and personel.
5. Immunosuppressive drug.
6. Relationship of KFU and NCCHD.


knst: PSC-Stroma

I was looking at it

I was looking at it. He was committed in the air. There was nothing wrong with it. Dive like that again and come back here in two weeks and play. Not again. Watch it.

I’m the referee on this field, not you. Stick to your job, and I will do mine. If I hear you shouting for anything again I’m gonna penalise you. This is not soccer.

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Aki ....  Akihiro Umezawa
Aki .... Akihiro Umezawa
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