Two MSCs lead cell-based therapy

My and PE slides

https://docs.google.com/spreadsheets/d/18my8KZ1l9kh5tXfKBXrxBs8vnvosPZ2oT4eGtL9tSoE/edit?usp=sharing

iPSC-hepatocyte

1. ReproCell

2. CDI $3,000---

3. Theratis

Ref. Primary hepatocytes: $1,5000--

HepaRG: $700---

Do iPSCs retain characteristics of their somatic origin?

In "Molecular Control of Induced Pluripotency, Cell Stem Cell, 14:720, 2014"

The question of whether iPSCs retain characteristics of their somatic origin has been a subject of considerable interest. While low-passage iPSCs may exhibit some epigenetic memory (Kim, et al., 2010), extended passaging brought the methylation patterns of iPSCs closer to ESCs (Nishino et al., 2011).

PLoS Genet. 2011 May;7(5):e1002085. doi: 10.1371/journal.pgen.1002085. 

DNA methylation dynamics in human induced pluripotent stem cells over time.

Nishino K1, Toyoda M, Yamazaki-Inoue M, Fukawatase Y, Chikazawa E, Sakaguchi H, Akutsu H, Umezawa A.

Antibody used for subcapsular implantation of HAES

Ab	Manufacturerer	Cat. No.	Web
Anti HLA　classⅠ-A, B, C, Mouse Monoclonal antibody	Hokudo co., ltd.	AB-46	http://www.hokudo.co.jp/shiyaku/reagent/HLAclassI.pdf
Human Albumin Antibody	Bethyl Laboratories, Inc.	A80-129A	https://www.bethyl.com/product/pdf/A80-129.pdf
Monoclonal  Mouse Anti-Human Ki-67 Antigen Clone MIB-1	Dako	M7240	http://www.dako.com/download.pdf?objectid=104980004
Anti-MRP2 antibody [M2 Ⅲ-6]	abcam	ab3373	http://www.abcam.com/mrp2-antibody-m2-iii-6-ab3373-references.html
Cytokeratin 19 (b170)	Leica	PA0799	http://www.leicabiosystems.com/ihc-ish-fish/novocastra-reagents/primary-antibodies/details/product/cytokeratin-19/
Anti-Cytochrome P450 3A4 antibody	abcam	ab135813	http://www.abcam.co.jp/cytochrome-p450-3a4-antibody-ab135813.html

PMD Act

Regulation to distribution by Nakayama: Is this correct?